FIG. 7.

Quantitative ELISAs for apoptosis of J774A.1 cells infected with one of several different P. aeruginosa strains. PAK, PAO1, and 388 are commonly used laboratory strains. 6294 is a hyperinvasive corneal isolate, and 617000 is a respiratory isolate. These strains were used to infect J774A.1 cells for 6 h at an MOI of approximately 160, after which cells were tested for evidence of apoptosis by using quantitative ELISAs. Strains varied markedly in their abilities to induce programmed cell death. Error bars represent standard errors of the means for experiments performed in triplicate.