Supplemental Figure S1.
Immunospecificity of the anti-phosphorylated Grk1 antibody (generated against the zebrafish phosphopeptide) towards phosphorylated GRK1 in mice. Adult wildtype and GRK1-S21A mice were dark-adapted overnight, euthanized, and retinal homogenates subjected to immunoblot analysis using the same novel zebrafish-specific antibody against phosphorylated zebrafish Grk1. Immunoblots were probed with antibodies against total Grk1 (green) and phosphorylated Grk1 (red) followed by incubation with secondary antibodies.