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. 2022 Oct 21;298(12):102636. doi: 10.1016/j.jbc.2022.102636

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Supplemental Figure S1 — Immunospecificity of the anti-phosphorylated Grk1 antibody (generated against the zebrafish phosphopeptide) towards phosphorylated GRK1 in mice. Adult wildtype and GRK1-S21A mice were dark-adapted overnight, euthanized, and retinal homogenates subjected to immunoblot analysis using the same novel zebrafish-specific antibody against phosphorylated zebrafish Grk1. Immunoblots were probed with antibodies against total Grk1 (green) and phosphorylated Grk1 (red) followed by incubation with secondary antibodies.