Figure 5.

Grk1 phosphorylation in rod grk1a and cone grk1b KO zebrafish. Immunoblots were probed with antibodies against total Grk1 (green) and phosphorylated Grk1 (red), followed by incubation with secondary antibodies. Comparison of Grk1 phosphorylation in (A) light- or dark-adapted WT and grk1b−/− larvae at 5 dpf, (B) light- or dark-adapted WT and grk1b−/− adults, and (C) light- or dark-adapted WT, grk1b−/−, and grk1a−/− larvae at 5 dpf. Black arrowhead: Grk1a; Black arrow: Grk1b; White arrowhead, phosphorylatedGrk1a. Panel B was spliced for clarity due to noncontiguous loading on the same gel.