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. 2022 Oct 27;15(11):1328. doi: 10.3390/ph15111328

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Fluorescence images of ki67 stained HDFs encapsulated in the hydrogels under different cell densities and cultivation models. (a) With 1.5 × 10⁶ of cell density. (b) With 3.0 × 10⁶ of cell density. The HDFs were stained for active proliferative nucleus (red; Ki67), and nucleus (blue; DAPI) under 20× magnification. (c) Percentage of active proliferative nucleus of HDFs for 1.5 × 10⁶ of cell density. (d) Percentage of active proliferative nucleus of HDFs for 3.0 × 10⁶ of cell density. * indicates p < 0.05.

Fluorescence images of ki67 stained HDFs encapsulated in the hydrogels under different cell densities and cultivation models. (a) With 1.5 × 10⁶ of cell density. (b) With 3.0 × 10⁶ of cell density. The HDFs were stained for active proliferative nucleus (red; Ki67), and nucleus (blue; DAPI) under 20× magnification. (c) Percentage of active proliferative nucleus of HDFs for 1.5 × 10⁶ of cell density. (d) Percentage of active proliferative nucleus of HDFs for 3.0 × 10⁶ of cell density. * indicates p < 0.05.