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Mutations within the β-strand twist motif of BoDV-1 P modulates viral replication. (a) Minireplicon assay using a series of P mutants. The relative luciferase activities are shown as the ratios relative to wild-type P transfected cells (WT). The indicated BoDV-1 P mutants were used as helper plasmids. The dots show mean values (biological replicates; n = 2). (b) Detection of BoDV-1 P mutants by western blotting. The whole western blot is shown in Figure S7.
