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. 2022 Nov 18;14(11):2553. doi: 10.3390/v14112553

Figure 3.

Figure 3

TMPRSS2 attenuated the inhibition of IFITM3. (A) HEK293T-ACE2 cells were transfected with IFITM3, TMPRSS2, or IFITM3 together with TMPRSS2 plasmid DNAs and then infected with lentiviral reporter viruses pseudotyped with SARS-CoV-2 spike protein. Infection efficiency was determined by luciferase activities and normalized to that of vector DNA-transfected cells. Expression of TMPRSS2, IFITM3, ACE2, and actin was measured by Western blotting. (B) Calu3 cells were transfected with siRNAs targeting IFITM3 (si2, si3) and then infected with lentiviral reporter viruses pseudotyped with SARS-CoV-2 spike protein. Infection efficiency was determined by luciferase activities and normalized to that of cells transfected with control siRNA (NC). Expression of IFITM3 and actin was measured by Western blotting. Data in the bar charts are presented as mean ± SD of three independent experiments. Statistical significance was determined with Student’s t test. ns, not significant; *, p < 0.05; ***, p < 0.001; NC, negative control; si2, IFITM3 siRNA2; si3, IFITM3 siRNA3.