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Antipolysaccharide antibody responses. Mice were immunized three times with 10 μg of Hib polysaccharide conjugated to rP40 or TT in presence of aluminum hydroxide. After one (white bars), two (horizontal bars), or three (black bars) immunizations, sera were collected and polysaccharide-specific IgG antibody content was measured by ELISA. Results are expressed as means ± SD (n = 5).
