Figure 1.

ROT2 expression and analysis of binary vector insertional events in Sporothrix schenckii wild-type and mutant strains. In (A), total RNA was isolated from the different mutant strains and used in RT-qPCR reactions to amplify a specific part of the ROT2 open reading frame. In (B), genomic DNA was isolated from the different strains and used in qPCR reactions that amplified the same DNA fragment used for gene expression analysis. In both cases, the amplification of the gene encoding the ribosomal protein L6 was used for data normalization. Data are means ± SD of three independent experiments performed in duplicates. * p < 0.05 when compared to the WT, HSS31, or HSS32 strains.