FIG. 6.
Recognition of mycobacterial antigens by mouse memory effector cells. Splenic lymphocytes were isolated from three memory immune mice 4 days after reinfection with 106 M. tuberculosis. The lymphocytes were stimulated in vitro with ST-CF (▴), nGroES purified by column chromatography (■), the recombinant fusion protein of the maltose binding protein and M. tuberculosis GroES (●), and the recombinant fusion protein treated with ST-CF (▾). The levels of IFN-γ were measured in culture supernatants; each point represents the mean of triplicate values ± standard error of the mean. Stimulation without antigen gave IFN-γ values below 0.05 ng/ml. This experiment was repeated twice with the same overall results.