TABLE 1.

Recognition of mycobacterial antigens by mouse memory effector cells^a

Expt	Antigenb	Mean IFN-γ releasec (ng/ml) ± SE
I	ST-CF	21.4 ± 0.9
	Ag85B	6.5 ± 0.2
	nGroES (I)	6.5 ± 0.2
	rGroES (Mt 10-2)	0.4 ± 0.1
	rGroES (Mt 10-1B)	0.3 ± 0.1
	rGroES (Ml 10-2)	0.3 ± 0.1
	rGroES (Ml 10-1B)	0.4 ± 0.2
II	ST-CF	22.9 ± 2.7
	nGroES (II)	3.2 ± 0.3
	rGroES (Mt 10-2)	0.4 ± 0.1
	rGroES (Mtcpn10)	0.5 ± 0.0
	sGroES	0.4 ± 0.0

^aSplenic lymphocytes were isolated 4 days after reinfection with 10⁶ M. tuberculosis and stimulated in vitro. 

^bAll antigens were used at a concentration of 5 μg/ml. nGroES (I) was purified by preparative electrophoresis; nGroES (II) was purified by column chromatography; rGroES (M. tuberculosis) was expressed in E. coli either in the pMAL system (Mt 10-2 and Mt 10-1B) or in the T7 expression system (Mtcpn10); rGroES (M. leprae) was expressed in the pMAL system (Ml 10-2 and Ml 10-1B); sGroES was the synthetic version of M. tuberculosis GroES. 

^cStimulation without antigen gave IFN-γ values of <0.05 ng/ml.