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. 2022 Nov 8;23(22):13742. doi: 10.3390/ijms232213742

Figure 2.

Figure 2

IN C-terminal tail sensing for TAR RNA apical stem-loop shape. (A) Structural model of TAR RNA and mutated versions used in our study: TAR long stem (TAR-LS, the red font underlines the added bases), TAR short stem (TAR-SS) and TAR very short stem (TAR-VSS). In order to verify that each RNA mutant assumed the expected secondary structure each model was analyzed with Fold WebServer [48,49]. (B) EMSA assay illustrating the interaction of IN-CTD and IN-CTD-ΔCT with TAR wild type and mutants. The RNA substrates (50 nM) are labelled with 32P (black star) and incubated with increasing concentrations of proteins (0; 100, 200, 400 and 800 nM) under the conditions described in Section 4.