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CLSM analysis of T. pyogenes isolate (BMH06-3) biofilm co-incubated with luteolin for 72 h (Green: live bacteria; red: killed bacteria; black: no bacteria). (a) Blank control biofilm. (b) Solvent control biofilm (1% DMSO). (c) Biofilm co-incubated with ceftiofur (0.25 ng/mL). (d–f) Biofilm co-incubated with luteolin (39, 78 and 156 μg/mL).
