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CLSM analysis of mature T. pyogenes (BMH06-3) biofilm disruption treated with 312 μg/mL luteolin (Green: live bacteria; red: killed bacteria; black: no bacteria). (a) Blank control biofilm. (b) Solvent control biofilm (1% DMSO). (c) Mature biofilm treated with ceftiofur (0.25 ng/mL) for 24 h. (d–l) Mature biofilm treated with 312 μg/mL luteolin for 2, 4, 6, 8, 10, 12, 16, 20 and 24 h.
