Figure 1.

Integration near specific non-B DNA is associated with proviral reactivation in latently infected cells. (A), Schematic depicting the HIV-1 vector used to generate productively and latently infected cells. (B), Strategy used in the Battivelli study to isolate productively infected cells (PIC), latently infected cells (LIC), reactivatable latently infected cells (RLIC), and non-reactivatable latently infected cells (NRLIC) cell populations. (C), Graphs show the percentage of total unique HIV-1 integration sites located in or within 500 bp of various non-B DNA motifs (distributed in 50 bp bins). Inset numbers show the percentage of total unique integration sites falling within 500 bp of the non-B DNA motif. Statistical analysis is with respect to PIC. (D), Heatmaps show the fold enrichment (blue) or depletion (red) of integration sites at each distance interval from the non-B DNA motif compared to the random control. The LIC population was compared to the PIC population. Fisher’s exact test; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Infinite number (inf), 1 or more integrations were observed when 0 integrations were expected by chance. Nan, not a number (0 integrations were observed and 0 were expected by chance). PIC (P), RLIC (R), NRLIC (N), LIC (L), LIC versus PIC (L vs P).