Table 2.
Overview of the lipopeptide production parameters including surfactin, iturin A or bacillomycin D and fengycin by B. velezensis UTB96 and B. velezensis FZB42 and in shake flask cultivations with initial glucose concentrations of 8 g/L, 20 g/L and 40 g/L and in a batch bioreactor using 40 g/L of glucose.
| Surfactin | Iturin A in UTB96 or Bacillomycin D in FZB42 |
Fengycin | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| B. velezensis |
Initial Glucose
[g/L] |
YX/S
[g/g] |
Growth Rate
µ [1/h] |
YP/S
[mg/g] |
YP/X
[mg/g] |
q
[mg/g.h] |
YP/S
[mg/g] |
YP/X
[mg/g] |
q
[mg/g.h] |
YP/S
[mg/g] |
YP/X
[mg/g] |
q
[mg/g.h] |
| UTB96 | 8 (SF) | 0.2 ± 0.01 |
0.2 ± 0.0 |
12.9 ± 1.16 |
27.4 ± 1.59 |
2.3 ± 0.13 |
5.8 ± 0.25 |
168.3 ± 8.76 |
1.2 ± 0.06 |
9.6 ± 0.13 |
281.5 ± 6.35 |
2.0 ± 0.04 |
| 20 (SF) | 0.6 ± 0.02 |
0.1 ± 0.0 |
3.6 ± 0.08 |
46.3 ± 5.93 |
0.6 ± 0.07 |
7.0 ± 0.86 |
134.4 ± 10.33 |
1.2 ± 0.09 |
3.4 ± 0.08 |
82.6 ± 1.41 |
0.7 ± 0.01 |
|
| 40 (SF) | 0.1 ± 0.01 |
0.1 ± 0.0 |
1.7 ± 0.1 |
62.4 ± 4.08 |
1.4 ± 0.09 |
3.5 ± 0.30 |
81.9 ± 9.51 |
0.6 ± 0.06 |
2.5 ± 0.13 |
55.4 ± 1.57 |
0,7 ± 0.01 |
|
| 40 (BR) | 0.6 ± 0.0 |
0.2 ± 0.0 |
8.4 ± 0.2 |
43.8 ± 3.8 |
0.9 ± 0.1 |
16.3 ± 0.8 |
249.4 ± 15.3 |
1.8 ± 0.0 |
1.2 ± 0.0 |
17.4 ±0.3 |
0.1 ± 0.0 |
|
| FZB42 | 8 (SF) | 0,2 ± 0.021 |
0.2 ± 0.00 |
5.0 ±0.24 |
120.6 ± 1.75 |
1.8 ± 0.00 |
1.4 ± 0 |
12.0 ± 2.04 |
0.3 ± 0.04 |
3.7 ± 0.59 |
81.9 ± 8.09 |
0.9 ±0.03 |
| 20 (SF) | 0.2 0.01 |
0.2 ± 0.01 |
1.2 ± 0.11 |
21.7 ± 1.68 |
0.3 ± 0.00 |
0.9 ± 0.01 |
35.2 ± 3.11 |
0.2 ± 0.02 |
1.3 ± 0.06 |
54.4 ± 6.23 |
0,3 ± 0.04 |
|
| 40 (SF) | 0.1 ± 0.0 |
0.1 ± 0.0 |
2.1 ± 0.04 |
39.6 ± 5.01 |
0.5 ± 0.06 |
0.4 ± 0.00 |
7.5 ± 0.53 |
0.1 ± 0.0 |
1.9 ± 0.4 |
32.1 ± 9.63 |
0.5 ± 0.14 |
|
SF—shake flask; BR—bioreactor.