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. 2022 Nov 21;14(11):2584. doi: 10.3390/v14112584

Figure 2.

Figure 2

Host-dependent pathogenicity of B.1.617.2 (Delta), WA1/2020 and B1.1.529 (BA.1) and BA.5 Omicron in hamster. (A) Schematic diagram of SARS-CoV-2 infection and experimental design. Interim Sac = scheduled interim sacrifice/euthanasia. Sac. = final euthanasia. Created with BioRender.com. Animals were intranasally inoculated with 5 × 103 TCID50/animal of SARS-CoV-2 WA1 or B.1.617.2/Delta or 6.5 × 105 TCID50/animal of BA.1 or BA.5 on day 0. Body weights before challenge were used to establish baseline. Survival, body weight changes (BWC), oral swabs, or nasal washes were observed or collected daily. Lungs were collected at the interim and terminal euthanasia on days 3 and 6 for WA1 and day 4 for B.1.617.2 (Delta), and BA.1 and BA.5 (Omicron). (B) Body weight change comparisons between WA1, B.1.617.2 (Delta), BA.1, and BA.5 for 4 dpi (and 5 dpi for WA1). (C) Viral titers estimated as genomic RNA copy number in lungs of infected mice at 3, 4, or 6 days postchallenge with 5 × 103 TCID50 of WA1 (n = 7), 5 × 103 TCID50 of B.1.617.2 (Delta, n = 7), or 6.5 × 105 TCID50 (n = 8) of BA.1 or BA.5 (n = 8). (D) Viral shedding profiles were estimated by RNA viral copy number in oral swabs collected from day 0 to day 5 dpi. Dpi = days postinfection. TCID50 = 50% of theTissue Culture Infectious Dose (TCID50).