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Antibody isotypes in sera of A/J, C57BL/6, BALB/c, and CD1 mice immunized with MAP4(SFPMNEESPLGFSPE)3P2P30 in TiterMax. Sera collected from mice 10 days after the third immunization (prior to challenge) were analyzed by ELISA against (SFPMNEESPLGFSPE)3, using heavy-chain-specific goat anti-mouse immunoglobulins as detecting antibodies. Data are shown as mean ± SD of the OD readings of quadruplicate assays.
