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. 2022 Nov 20;27(22):8063. doi: 10.3390/molecules27228063

Figure 2.

Figure 2

SDS-PAGE analyses of reaction solutions of the auto-processing of proCCN (a), [K63G]-proCCN (b), proCCN’ (c), and [K8D]-proCCN’ (d). Lanes 1 and 2: 0 min and 16 h at 4 °C, respectively; lanes 3–8: 0 min, 1 h, 3 h, 24 h, 48 h, and 72 h at 25 °C, respectively (a–c). Lanes 1 and 2: 0 min and 24 h of proCCN’, respectively; lanes 3 and 4: 0 min and 24 h of [K8D]-proCCN’, respectively (d). Black, gray, and red arrowheads indicate proCCN proteins, degradation products, and CCN proteins, respectively. Blue arrowhead indicates the degradation product that is cleaved at the Lys8 residue.