Histopathologic examination of lung and trachea lesions in mice at sixth day post-challenge of H3N2 (CVCC AV1520). (A–F): Lung changes of different groups. (A): NC (lung); (B): inactivated vaccine (IIV) group; (C): BNV group; (D): BNV + MF59 group; (E): BNV + CPG group; (F): PBS control group; (G–I): trachea changes of different groups; (G): NC (trachea); (H): inactivated vaccine (IIV) group; (I): BNV group (Scale bar = 100 µm). All specimens were processed according to the SOP procedure for pathological examination. Dehydration, pruning, embedding, sectioning, staining, sealing, and microscopic examination.