Table 2.
Top ranked putative novel AMR genes in K. pneumoniae, not yet implicated as carbapenem resistant genes, following the verification of their presence in the resistant strains for each antibiotic (Doripenem, Ertapenem, Imipenem, and Meropenem). These novel AMR genes appeared among top ranked genes of importance by machine learning in each round of the 6-fold cross validation for K. pneumoniae.
| SN. | Doripenem | Ertapenem | Imipenem | Meropenem | ||||
|---|---|---|---|---|---|---|---|---|
| Accession | Protein Name/Description | Accession | Protein Name/Description | Accession | Protein Name/Description | Accession | Protein Name/Description | |
| 1 | WP_004144294.1 | AlpA family phage regulatory protein | WP_040215863.1 | (2,3-dihydroxybenzoyl)adenylate synthase EntE | WP_004152650.1 | DUF1833 family protein | WP_004152389.1 | DUF2913 family protein |
| 2 | WP_048999684.1 | Cu(+)/Ag(+) sensor histidine kinase | WP_016338366.1 | DUF2913 family protein | WP_004218558.1 | DUF2560 family protein | WP_071182194.1 | DUF551 domain-containing protein |
| 3 | WP_000085883.1 | DNA methylase | WP_000454193.1 | DUF3330 domain-containing protein | WP_004152389.1 | DUF2913 family protein | WP_004184301.1 | DinB family protein |
| 4 | WP_004218558.1 | DUF2560 family protein | WP_004171440.1 | hypothetical protein | WP_062955115.1 | GABA permease | WP_004199358.1 | hypothetical protein |
| 5 | WP_032427884.1 | hypothetical protein | WP_004151678.1 | phosphodiester glycosidase family protein | WP_015065545.1 | hypothetical protein | WP_004217321.1 | IS6-like element IS26 family transposase |
| 6 | WP_023282973.1 | arsenic transporter | WP_000219391.1 | Mph(A) family macrolide 2’-phosphotransferase | WP_023300766.1 | L-2-hydroxyglutarate oxidase | WP_071557814.1 | IS6-like element IS6100 family transposase |
| 7 | WP_004144136.1 | bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE | WP_004141234.1 | NAD(P)-binding domain-containing protein | WP_004199843.1 | MetQ/NlpA family ABC transporter substrate-binding protein | WP_015367306.1 | MAPEG family protein |
| 8 | WP_004152357.1 | DNA polymerase III subunit theta | WP_139153029.1 | Tn3 family transposase | WP_023290921.1 | PD40 domain-containing protein | WP_032706418.1 | PTS-dependent dihydroxyacetone kinase operon transcriptional regulator DhaR |
| 9 | WP_000997453.1 | type VI secretion protein | WP_016240610.1 | TraM recognition domain-containing protein | WP_068815603.1 | PTS-dependent dihydroxyacetone kinase operon transcriptional regulator DhaR | WP_000166328.1 | Rop family plasmid primer RNA-binding protein |
| 10 | WP_039817891.1 | IS21 family transposase | WP_064162557.1 | UDP-N-acetylmuramate dehydrogenase | WP_015703570.1 | YqjK-like family protein | WP_019725280.1 | SAM-dependent DNA methyltransferase |
| 11 | WP_000018330.1 | aminoglycoside O-phosphotransferase APH(3’)-Ia | WP_004175424.1 | YebG family protein | WP_004150845.1 | dimethylsulfoxide reductase subunit B | WP_015365583.1 | alanine transaminase AlaA |
| 12 | WP_004152392.1 | IS3-like element Tn4401 family transposase | WP_002901627.1 | YniB family protein | WP_009485658.1 | glycerol dehydratase reactivase beta/small subunit family protein | WP_015365705.1 | arsenic transporter |
| 13 | WP_004218009.1 | glycosyl hydrolase family 2 | WP_002888321.1 | bis(5’-nucleosyl)-tetraphosphatase (symmetrical) | WP_020802362.1 | multidrug/biocide efflux PACE transporter | WP_015704003.1 | bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA |
| 14 | WP_004198233.1 | zinc-binding domain of primase-helicase family protein | WP_004152235.1 | dimethylsulfoxide reductase subunit B | WP_000843500.1 | DUF2933 domain-containing protein | WP_015703802.1 | deacetylase |
| 15 | WP_001010162.1 | arsenical pump-driving ATPase | WP_004191677.1 | helix-hairpin-helix domain-containing protein | WP_009483845.1 | SLC13/DASS family transporter | WP_000361389.1 | plasmid partitioning/stability family protein |