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. 2022 Oct 31;14(11):747. doi: 10.3390/toxins14110747

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Reactivity of polyclonal antibodies (PoAbs) after each booster immunization with nivalenol (NIV), as determined via a direct competitive enzyme-linked immunosorbent assay (dc-ELISA). Green, orange, and blue lines show the mean values for reaction of the PoAbs from three mice after the 1st, 2nd, and 3rd booster immunizations, respectively. B/B₀ = ratio of absorbance with NIV to absorbance without NIV. Each data point is the mean of three replicates based on independent examinations; error bars indicate ± SD.