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. 2022 Nov 17;23(22):14215. doi: 10.3390/ijms232214215

Figure 3.

Figure 3

The R930H variant induces transcript variant dependent gating changes of Cav1.3 channels. (a) Representative current traces of wild-type Cav1.3S and mutant Cav1.3S R930H (R930HS) or (b) wild-type Cav1.3L and mutant Cav1.3L R930H (R930HL) upon a depolarization step to the potential of their maximal inward currents. (c,d) Current-voltage relationships of the ICa (mean ± S.E.M.) of (c) wild-type versus mutant short and (d) long Cav1.3 transcript variants. Note that the R930H variant enhanced current densities in the short splice variant. In contrast, current densities were reduced in the mutant long splice transcript variant. (e) Boxplots of the peak current densities obtained from depolarization to the respective voltage of the peak current (displayed as median (dotted lines), 25/75 percentile (whiskers) and mean (black lines)); median and 25/75 percentiles. In pA/pF: wild-type Cav1.3S: −6.8 ± (−10.5/−5.7), n = 32 and R930HS: −11.3 ± (−23.7/−7.7), n = 39, p = 0.0016; wild-type Cav1.3L: −11.2 ± (−19.1/−6.4), n = 55 and R930HL: −9.5 ± (−10.4/−7.2), n = 32, p = 0.14: **: p < 0.01. Statistics were performed using the Mann–Whitney test for both splice variants.