Table 2.
Comparison of activation and inactivation parameters of wild-type (native) Cav1.3 and the Cav1.3 R930H channels.
| Activation | Inactivation | ||||||
|---|---|---|---|---|---|---|---|
| Vrev [mV] | Slope [mV] | V0.5 [mV] | n | V0.5 [mV] | Slope [mV] | n | |
| Cav1.3S | 67.62 ± 1.05 | 7.66 ± 0.11 | −8.94 ± 0.41 | 50 | −29.69 ± 1.06 | 4.45 ± 0.16 | 15 |
| R930HS | 67.63 ± 1.27 | 7.17 ± 0.10 ** | −8.67 ± 0.43 | 39 | −30.24 ± 1.00 | 4.47 ± 0.23 | 9 |
| Cav1.3L | 69.35 ± 0.65 | 9.24 ± 0.11 | −0.14 ± 0.56 | 79 | −25.72 ± 2.08 | 5.56 ± 0.23 | 18 |
| R930HL | 68.80 ± 0.82 | 9.24 ± 0.16 | 1.73 ± 1.06 | 32 | −24.11 ± 1.12 | 6.22 ± 0.35 | 12 |
Activation and inactivation parameters were obtained after fitting normalized G/V relationships or normalized steady-state inactivation curves, as described in the methods section. Data are shown as mean ± S.E.M. from more than three independent transfections. ** = p < 0.01 versus Cav1.3S using unpaired Student’s t-test. Long and short splice variants were analyzed separately. V0.5, voltage of half-maximal activation/inactivation; Vrev, reversal potential; n, number of experiments. For composition of Cav1.3 transcripts, see Section 4.