Table 1.

Primer and probe sequences that were used in VZV multiplex.

Gene	Primer/Probe Sequence	Reference
Human GAPDH_F	5’-CACATGGCCTCCAAGGAGTAA-3’	[46]
Human GAPDH_R	5’-TGAGGGTCTCTCTCTTCCTCTTGT-3’
Human GAPDH_Probe	5’-(Cy5)CTGGACCACCAGCCCCAGCAAG(IAbRQSp)-3′
VZV ORF23_F	5′-CTTCTGGACAACAACCGCAA-3′	n/a
VZV ORF23_R	5′-CAGATTGTCCCGTGTGTGAC-3′
VZV ORF23_Probe	5′-(TexRed-XN)-ACTGTCCAGCCAACAACCGG-(IabRQSp)-3′
VZV ORF62_F	5′-CCTTGGAAACCACATGATCGT-3′	[47]
VZV ORF62_R	5′-AGCAGAAGCCTCCTCGACAA-3′
VZV ORF62_Probe	5′-(HEX)-TGCAACCCGGGCGTCCG-(ZEN/IabRQSp)-3′
VZV ORF63_F	5′-GATGGTGGTGAAGACGA-3′	n/a
VZV ORF63_R	5′-AATCGGTGCTCTCCTCT-3′
VZV ORF63_Probe	5′-(FAM)-CGGAATCATCGGACGGGGAAG-(ZEN/IabRQSp)-3′
VLT 3-4_F	5′-AATCGAGCCATACACCACCG-3′	[24]
VLT 3-4_R	5′-TGTATTCGGGCATGGACCTC-3′
VLT 3-4_Probe	5′-(FAM)-GATCGAACAGCAGATGGATTGCA-(ZEN/IabRQSp)-3′