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. 2022 Oct 25;8(11):1123. doi: 10.3390/jof8111123

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phylogenetic tree based on TUB2 barcode. Genetic diversity and taxonomic assignment of the studied Colletotrichum isolates against accessions in the NCBI database and assessed by DNA barcode for the beta-tubulin (TUB2) locus. The Colletotrichum clades coccodes, gloeosporioides, and acutatum are depicted by grey, red, and green boxes, respectively. The trees were constructed in MEGA11 using the UPGMA clustering method and the Kimura 2-parameter model. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) is shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. All ambiguous positions were removed for each sequence pair (pairwise deletion option). Sequences derived from the NCBI are shown with their accession number and organism name.