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Efficiency of PCR amplification and sequencing for Colletotrichum specimens for four DNA barcode regions.
| Barcode Region | N | Alignment Length (bp) | % AE | % SE |
|---|---|---|---|---|
| ITS | 26 | 556 | 100 | 100 |
| ACT | 26 | 246 | 100 | 100 |
| EF-1a | 19 | 242 | 75.0 | 100 |
| Tub2 | 26 | 750 | 100 | 100 |
N = samples with successful amplification, % AE = Percentage of amplification efficiency, % SE = percentage of sequencing efficiency (from the amplified barcodes).
