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. 2022 Nov 22;2022:gigabyte73. doi: 10.46471/gigabyte.73

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© The Author(s) 2022.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Phylogenetic analysis of cytochrome oxidase 1 (CO1) genes for Diadema and Echinothrix spp.

A maximum likelihood bootstrapped consensus tree is shown for 15 partial coding sequences of Diadema and Echinothrix spp. plus Eucidaris tribuloides as an outgroup. A section of the mitogenome for E. diadema (KX385836), E. calamaris (KNZ-KUC07), D. antillarum (10.02.2022) and E. tribuloides (ETRI 00-1) that corresponded to the CO1 gene was extracted by generating an initial alignment. To do this, the E. calamaris gene-specific sequences listed in the tree were aligned to the entire E. tribuloides, E. diadema and E. calamaris mitogenome. Separately, the gene-specific sequences listed in the tree from D. savignyi, D. antillarum, and D. setosum were aligned to the entire E. tribuloides and D. antillarum mitogenome. The rest of the mitogenome for each species was trimmed to only reflect the portion pertaining to the CO1 gene sequence. Thus, the taxa on the tree named Diadema antillarum mtDNA 10.02.2022, Echinothrix calamaris KNZ-KUC07 MK609484=NC050274, Echinothrix diadema KX385836=NC 033523 and Eucidaris tribuloides isolate ETRI 00-1 MH614961=NC 050252 include only the CO1 gene sequences. Branch lengths are equalized and do not include evolutionary divergence times. Bootstrap support values are included on the tree branches.