Figure 1.

The effect of (a) IND2 on the proliferation of (b) DU145 and PC3. (c) IC₅₀ values for PC3 and DU145 cells. The results are represented as the means ± SEM of three independent experiments performed in triplicate. (d) The effect of IND-2 (2.5 and 10 µM) on the colony formation rate in PC3 cells. (e) Morphological changes induced by IND-2 on PC3 cells after incubation with vehicle and IND-2 (5 and 10 µM) for 0, 48 and 72 h. Biotek Cytation 7 was used to capture the images. (f) A representative confluence curve for PC-3 cells incubated with vehicle and IND-2 (0, 1.25, 2.5, 5 or 10 µM).