Figure 5.

IND-2 induces apoptosis in PC-3 prostate cancer cells. (a) Western blots for the proteins involved in the induction of apoptosis, cleaved caspases 3/7 and cleaved PARP, from total cell lysates incubated with vehicle (negative control), IND-2 (1 and 5 µM) and paclitaxel (0.5 µM), for 24 h. (b) DNA ladder assay was performed using agarose gel electrophoresis to visualize DNA fragmentation which preceded apoptotic morphological changes. Cells incubated with IND-2 (1 and 5 µM) were harvested and subjected to DMSO DNA extraction. Two thousand ng DNA was run on a 1.5% agarose gel and visualized for fragmentation. IND-2 induced DNA fragmentation as early as 8 h post-incubation ‘B’ indicates blank wells. (c) A histogram summarizing the proteins quantified from the western blots using Image J. The data represent means ± SD of two independent experiments (* p < 0.05).