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. 2022 Nov 14;23(22):14051. doi: 10.3390/ijms232214051

Figure 2.

Figure 2

Purification and Uv-Vis spectroscopy of Sox9 construct. (a) Sox9HMG-sox_N construct (amino acids 1–173) was expressed in bacteria and purified using Ni-IMAC affinity chromatography, owing to having 6xHis at N-terminal of the construct. (b) Protein was concentrated and subsequently purified using gel filtration chromatography. The gel filtration chromatogram showed a homogenous and monodisperse peak. (c) SDS-PAGE of the gel filtration elution fractions corresponding to the Sox9HMG-sox_N construct. (d) Uv-Vis spectroscopic spectra of the purified Sox9HMG-sox_N showing absorption maxima between 400–450 nm. Buffer was used as a reference.