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. 1999 Nov;67(11):5676–5682. doi: 10.1128/iai.67.11.5676-5682.1999

FIG. 2.

FIG. 2

PknB expression. (A) SDS-PAGE analysis of PknB expression in E. coli. Lanes: 1, molecular size markers; 2, negative control pET-22b in E. coli BL21 after IPTG induction; 3, cell extract of pYA102 expressing PknB after IPTG induction; 4, pellet of cell extract of pYA102 expressing PknB after IPTG induction; 5, PknB inclusion bodies after washes with detergent; 6, PknB after urea-DTT treatment; 7, PknB after size-exclusion chromatography. (B) Western analysis using mouse anti-PknB polyclonal antibodies. Lanes: 1, negative control pET-22B; 2, pellet of cell extract of pYA102 expressing PknB after IPTG induction; 3, PknB inclusion bodies; 4, PknB after urea-DTT treatment; 5, PknB after size-exclusion chromatography.