FIG. 4.

PknB cross-reactivity with eukaryotic phosphoprotein antibodies and phosphoamino acid analysis. (A) Cross activity of PknB with antibodies against phosphoproteins was determined by SDS–7.5% PAGE analysis followed by Western blot analysis. (B) Phosphoamino acid analysis of autophosphorylated PknB was performed by excision of radioactively labelled PknB from a PVDF membrane followed by acid hydrolysis and two-dimensional thin-layer chromatography. The positions of the unlabeled phosphoamino acids standards are encircled.