Table 3.
Properties and kinetic parameters of LOXL2 in cadaverine oxidation.
| LOXL2 (0.03 μM) |
LTQ (%) |
Cu2+ (%) |
kcat (min−1) |
Km (mM) |
kcat/Km (min−1mM−1) |
Relative kcat/Km (%) |
|---|---|---|---|---|---|---|
| WT | 100 | 0.030 (100) | 9.28 ± 0.21 | 0.92 ± 0.07 | 10.09 ± 0.80 | 100 |
| H623Q | <50 | 0.029 (97) | 1.28 ± 0.15 | 0.06 ± 0.22 | 2.13 ± 0.82 | 21 |
| H626Q | 0 | 0.006 (20) | 0 | − | − | − |
| H628Q | 0 | 0.004 (13) | 0 | − | − | − |
| H630Q | 0 | 0.011 (37) | 0 | − | − | − |
| H637Q | 90 | N.D. | 3.51 ± 0.26 | 0.48 ± 0.12 | 7.31 ± 1.91 | 72 |
| H652Q | <50 | 0.016 (53) | 3.06 ± 0.14 | 0.78 ± 0.10 | 3.92 ± 0.53 | 39 |