Table 1.

Mycotoxin extraction from human sera results in repeatable (CV%) recovery percentages for all analytes. The recovery percentage refers to the log10 concentration (ng/mL or ppb) of analyte recovered from spiked samples before versus after extraction. Matrix effect (ME) and matrix factor (MF) help to determine the influence of human sera on recovery percentages. Ideally, MF will equal 1, and ME should be roughly 0% to indicate that the extraction process is neither suppressing nor enhancing the recovery results.

Mycotoxin	Spike (ppb)	Mean Recovery (%)	Mean Repeatability (CV%)	Mean Matrix Effect (%)	Mean Matrix Factor
AFB1	0.61	96	0.05	−72.11	0.27
	2.44	97	4	−65.91	0.33
	9.77	96	4	−49.71	0.48
	39.06	101	2	−40.50	0.60
DON	19.53	98	4	−9.28	0.88
	78.13	94	5	−0.78	0.93
	312.50	94	7	6.19	1.00
	1250	91	17	8.75	0.99
FUM B1/B2	4.88	73	0.1	5.47	0.77
	19.53	81	11	−0.44	0.80
	78.13	79	6	3.12	0.81
	312.50	91	2	−3.76	0.88
OTA	19.53	81	0.4	−9.51	0.74
	78.13	86	21	−10.16	0.76
	312.50	79	3	−3.69	0.76
	1250	92	6	−7.43	0.85
ZEA	0.15	85	4	−39.22	0.52
	0.61	95	3	−29.88	0.66
	2.44	89	10	−12.69	0.78
	9.77	106	18	−10.12	0.94