Figure 2.

Phenotypic changes of WT, bdx-1, and OEBDX seedlings during thermotolerance and thermomemory. (A) WT Col-0, bdx-1, and OEBDX seedlings were grown on MS medium. Seedlings with two leaves were subjected to priming treatment (T₀P). Seedling phenotypes of control, 7d post priming and 3d post priming, and 4d post triggering are shown. (B) The bleaching cotyledon phenotype was quantified in seedlings shown in (A) and represented as a percentage. (C) Leaves observed at 4d post triggering. Error bars indicate SEM (n = 3). An amount of 10–12 seedlings constituted each biological replicate. For cotyledon bleaching, statistical analyses were performed using arcsine conversion of percentages. Different letters indicate significant differences calculated using one-way ANOVA with post-hoc Tukey’s test (p < 0.05).