Supplemental Figure S1.

Manufacturer-dependent FPR activation by Aβ1-42 A, representative TEM images of Aβ1-42 obtained from either Peptides&Elepehants (P&E), Synpeptide, or Anaspec show different types of aggregates. AS-HFIP refers to a commercially available HFIP-treated peptide from Anaspec. Scale bars indicate 0.5 μm. B, mean Ca2+ peak responses of FPR-transfected HEK293T cells after stimulation with 10 μM Aβ1-42 obtained from different manufacturers. Colored bars indicate the response of human (red) or mouse (blue) FPRs, n=3, N=2. Data correspond to the heat map shown in Fig 2B. Bar graphs for P&E and Synpeptide are shown again for the sake of comparability. C, complete CD spectra of all four Aβ1-42 displayed in figure 2. N=3. All Error bars, S.D.; One-way ANOVA test, Dunnett post hoc test; ∗, p ≤ 0.05; ∗∗, p ≤ 0.01; ∗∗∗, p ≤ 0.001; ns, no significance