Supplemental Figure S6.

FPR1 is the dominant receptor in U87 cells. A, Ct values of all human FPRs from the RNA of U87 cells obtained through RT-qPCR, n=5, N=2. B, Ca2+ responses of U87 cells (green) or FPR-transfected HEK293T cells (red) towards 5 μM Aβ17-40 with (clear bars) or without (striped bars) co-application of 10 μM tBoc2. Signals were normalized to responses of the respective cells towards 10 μM WKYMVm, n=1, N=2 for HEK293T cells and N=3 for U87 cells. C, mean Ca2+ peak responses of U87 cells (green) or FPR-transfected HEK293T cells (red) that were treated with 10 nM SP4 (left) or 10 nM SP6 (right), n=1, N=3. All Error bars, S.D. One-way ANOVA test, Dunnett post hoc test; ∗, p ≤ 0.05, ∗∗, p ≤ 0.01, ∗∗∗, p ≤ 0.001; ns, no significance