Supplemental Figure S7.

Validation of RT-qPCR primers for all human FPRs. Left: mean standard curves, regression line, and amplification efficiency (n =3, N=3) generated by using a 10-fold serial dilution of a target DNA template starting with 0,1 ng of a purified and sequenced PCR product in t-RNA. Ct values were plotted against the log of template quantity for each dilution. Representative PCR products for all primer sets were controlled by gel electrophoresis and sequencing for their specificity. Right: A representative data set of an amplification curve from these dilution series for each primer.