Supplemental Figure S8.

Evidence for similar cell surface expression and calcium responses of FPR1 and FPR2 in HEK293T cells. A, HEK293T cells were transfected with varying amounts of plasmid DNA for hFPR1 or hFPR2 and subsequently treated with 1 μM of FITC-labeled WKWVm-NH2 to monitor their cell surface expression. Left: representative images of treated cells transfected with different DNA amounts Right: quantitative analysis of positively stained cells. Note that cell surface expression of both receptors upon dilution is reduced in a similar manure. Error bars, S.D. n=1, N=3 B, Mean Ca2+ peak responses of HEK293T cells transfected with decreasing amounts of human (red) or mouse (blue) FPR plasmid DNA. FPR1 and 2 of each species were treated with 1 μM of WKWVm-NH2 and FPR3-transfected cells with 30 μM of WKWVm-CHO. Note that the amount of FPR 1 and 2 DNA can be diminished by more than 10fold without a strong reduction of the signal amplitudes.