Figure 4.

N-abridged Aβ peptides induce hFPR1-dependent responses in glial U87 cells.A, mean Ca²⁺ peak responses of U87 cells after stimulation with different concentrations of Aβ_11-40 or Aβ_17-40.Striped bars indicate the response towards 10 μM of the positive control WKWVm-NH₂, light gray and dark gray bars indicate negative controls; n = 3, N = 1. B, comparison of the Ca²⁺ responses upon stimulation with either 5 μM Aβ_11-40 or Aβ_17-40 alone (green bars) or in the presence of 10 μM of the competitive FPR-antagonist tBoc2 (black bars); n = 3, N = 1. C, dose-dependent chemotaxis of U87 cells upon stimulation with either Aβ_11-40 or Aβ_17-40. Each bar represents the number of cells that migrated through a porous membrane towards the respective stimuli. Green bars indicate migration towards N-abridged fragments, striped bars display migration towards the positive control 1 μM fMLF, light gray bars show migration without stimuli, and dark gray bars represent the response to 0.1% DMSO, n = 3, N = 1. D, migration of U87 cells that were either untreated (green bars) or treated with 10 μM tBoc2 (black bars) towards 1 μM of Aβ_11-40 or Aβ_17-40; n = 3, N = 1. All Error bars, S.D. One-way ANOVA test, Dunnett post hoc test for A and C and t test for B and D; ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001; ns, no significance. Aβ, amyloid beta; FPR, Formyl peptide receptor.