FIG. 1.

Plasmid profiles of B. burgdorferi isolates and subclones of isolate 225c. (A) Plasmid-enriched DNA was analyzed on a 0.2% agarose gel and stained with ethidium bromide. Lanes: 1 and 2, isolates 225a and 225c, respectively, which were cultured sequentially from a P. leucopus mouse; 3 and 4, isolates from mice C.B-17 1 and 2 inoculated 1 month previously with 225a and 225c and which served to infect larval I. scapularis ticks; 5 and 6, isolates from C3H 1 and 2 obtained 1 month after nymphal tick feeding; 7 and 8, subclones 2E7′ and 3B6′ obtained from 225c by limiting dilution. DNA molecular mass standards are indicated on the left. (B) Plasmid DNA was transferred to nylon membrane and was hybridized to pBHB63 (specific for the 16-kb linear plasmid) labeled by ECL. The gel photograph and hybridization were scanned into Adobe Photoshop 3.1, scaled to the column width, and labeled.