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Effect of compound C1 on human and mouse melanoma cell lines. Human (A375, upper panels) and mouse (B16, lower panels) melanoma cells were seeded into a 48-well plate overnight and treated with C1 for 48 h. Cell viability was determined by MTT reductase activity (photographs) and trypan blue (TB) exclusion assay (bar graphs) after exposure to hit compound C1 (shown here at a concentration of 11 µg/mL for human cells and 2.75 µg/mL for mouse cells). * Statistically significant.
