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. 2022 Oct 31;15(11):1347. doi: 10.3390/ph15111347

Figure 5.

Figure 5

Effect of kushenol F on the serum histamine level and immunoglobulins. (A) Histamine levels were detected using a fluorescent plate reader. (B) Serum IgE, (C) serum IgG2a and (D) DFE-specific IgE concentrations were quantified using ELISA. The blood samples of the vehicle, DFE/DNCB + vehicle and DFE/DNCB + kushenol F (2, 10 or 50 mg/kg) groups were collected from the facial vein at 28 days. Data are presented as the mean ± SD (n = 5). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control. DFE, Dermatophagoides farinae extract; DNCB, 1-chloro-2,4-dinitrochlorobenzene.