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TM-induced ERS effects on ubiquitin-related genes and ubiquitin-protein in piglets. Piglets were administrated with TM LD (0.1 mg/kg body weight), HD (0.3 mg/kg body weight), or vehicle for 48 h. (A–D): Relative mRNA levels of UBA2, UBA3, UBE2E, and UBE2I were assessed using real-time RT-PCR in different groups. (E): Relative protein levels of ubiquitin and β-actin were assessed using Western blot. (F): Western blot bands. * means p < 0.05; ** means p < 0.01.
