Figure 9.

Effects of GRP94 knockdown on ubiquitin-related genes and ubiquitin proteins in immortalized porcine HSCs. (A–D): Relative mRNA levels of UBA2, UBA3, UBE2E and UBE2I in immortalized HSCs were measured using real-time RT-PCR. (E): Relative protein levels of ubiquitin in immortalized HSCs were assessed using Western blot. (F): Western blot bands. Data were assessed with two-way ANOVA (main effects: ERS, shRNA, and their interaction), followed by Bonferroni’s multiple comparison post hoc test; the same letters mean no significant difference (p < 0.05) between groups.