Skip to main content
. 2022 Nov 14;23(22):14059. doi: 10.3390/ijms232214059

Figure 10.

Figure 10

GRP94 knockdown effects on immortalized porcine HSCs apoptosis. (A–C): TUNEL staining of immortalized HSCs observed under a 200× objective lens. (D–F): Cell proliferation assay of immortalized HSCs after TM treatment for 24 h, 36 h and 48 h by CCK-8 assay. (G,H): Relative protein levels of Bcl-2 and Caspase 3 in immortalized HSCs were assessed using a Western blot. (K): Western blot band. (I,J): Relative mRNA levels of Bcl-2 and Caspase 3 in immortalized HSCs were measured using real-time RT-PCR. Data were assessed with two-way ANOVA (main effects: ERS, shRNA, and their interaction), followed by Bonferroni’s multiple comparison post hoc test; the same letters mean no significant difference (p < 0.05) between groups.