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. 2022 Nov 11;23(22):13897. doi: 10.3390/ijms232213897

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterisation of human dental pulp stem cells (hDPSCs). (A) The surface marker expression was analysed using flow cytometry. Multilineage differentiation potential to (B) osteogenic and (C) adipogenic lineage. Scale bars: 300 (B) and 30 (C) µm, respectively. The hDPSCs were cultured in an osteogenic differentiation medium (OM) and adipogenic differentiation medium (ADM). The mineral deposition was stained using Alizarin Red S (ARS) staining. The accumulation of lipid droplets in the cytoplasm was stained by Oil Red O staining.