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. 2022 Oct 27;14(11):736. doi: 10.3390/toxins14110736

Figure 7.

Figure 7

Correlation of LC-UV and MS chromatogram and bioassay chromatogram of N. pallida venom. (A) UV chromatogram of N. pallida venom (1 mg/mL, 50 μL injection volume, post-column split into 1:9 ratio of which the smaller portion went to UV (220 and 254 nm recorded); and then to MS detection. Superimposed bioassay chromatograms resulting from analyses of N. pallida venom (0.2 mg/mL, 50 µL injection volume) in the presence of different concentrations of (B) varespladib. (C) Superimposed anticoagulation bioassay chromatograms resulting from analyses of several diluted N. pallida venom samples ranging from 1 mg/mL to 0.008 mg/mL (50 µL per injection). (D) Base peak chromatogram (BPC) and extracted ion currents (XIC) mass spectrometry. Bioassay chromatograms are correlated to UV and MS. Proteomic annotation of N. pallida venom using Mascot software against (E) the Swiss-Prot database and (F) species-specific venom gland transcriptomic-derived database. The protein score represents the probability that designated proteins, defined by elution time, are present in the sample.