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. 2022 Oct 27;14(11):736. doi: 10.3390/toxins14110736

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Correlation of LC-UV and MS chromatogram and bioassay chromatogram of N. haje venom. (A) UV chromatogram of N. haje venom (1 mg/mL, 50 μL injection volume, post-column split into 1:9 ratio of which the smaller portion went to UV (220 and 254 nm recorded); and then to MS detection. Superimposed bioassay chromatograms resulting from analyses of N. haje venom (0.2 mg/mL, 50 µL injection volume) in the presence of different concentrations of (B) varespladib. (C) Superimposed anticoagulation bioassay chromatograms resulting from analyses of several diluted N. haje venom samples ranging from 1 mg/mL to 0.008 mg/mL (50 µL per injection). (D) Base peak chromatogram (BPC) and extracted ion currents (XIC) mass spectrometry. Bioassay chromatograms are correlated to UV and MS. Proteomic annotation of N. haje venom using Mascot software against (E) the Swiss-Prot database and (F) species-specific venom gland transcriptomic-derived database. The protein score represents the probability that designated proteins, defined by elution time, are present in the sample.